A simple method is reported to fabricate gold arrays featuring microwells surrounding 8-electrodes from gold compact discs (CDs) for less than $0.2 per chip. Integration of these disposable gold CD array chips with microfluidics provided inexpensive immunoarrays that were used to measure a cancer biomarker protein quickly at high sensitivity. The gold CD sensor arrays were fabricated using thermal transfer of laserjet toner from a computer-printed pattern followed by selective chemical etching. Sensor elements had an electrochemically addressable surface area of 0.42 mm2 with RSD \u3c2%. For a proof-of-concept application, the arrays were integrated into a simple microfluidic device for electrochemical detection of cancer biomarker interleukin-6 (IL-6) in diluted serum. Capture antibodies of IL-6 were chemically linked onto the electrode arrays and a sandwich immunoassay protocol was developed. A biotinylated detection antibody with polymerized horseradish peroxidase labels was used for signal amplification. The detection limit of IL-6 in diluted serum was remarkably low at 10 fg mL −1 (385 aM) with a linear response with log of IL-6 concentration from 10 to 1300 fg mL −1. These easily fabricated, ultrasensitive, microfluidic immunosensors should be readily adapted for sensitive detection of multiple biomarkers for cancer diagnostics.
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机译：据报道，一种简单的方法可以用金光盘（CD）制造具有围绕8电极的微孔的金阵列，每个芯片的价格不到0.2美元。这些一次性金CD阵列芯片与微流控技术的集成提供了廉价的免疫阵列，可用于以高灵敏度快速测量癌症生物标记蛋白。金的CD传感器阵列是使用激光喷出的碳粉从计算机打印的图案进行热转印，然后进行选择性化学蚀刻制成的。传感器元件的电化学可寻址表面积为0.42 mm2，RSD为u3c2％。对于概念验证应用，将阵列集成到简单的微流控设备中，用于电化学检测稀释血清中的癌症生物标记白介素-6（IL-6）。将IL-6的捕获抗体化学连接到电极阵列上，并开发了一种三明治免疫测定方案。具有聚合辣根过氧化物酶标记的生物素化检测抗体用于信号放大。稀释血清中IL-6的检出限非常低，为10 fg mL -1（385 aM），线性响应，IL-6浓度从10到1300 fg mL -1的对数。这些易于制造的超灵敏微流体免疫传感器应易于适应多种生物标志物的灵敏检测，以用于癌症诊断。
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